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Standard compounds 2′-deoxycytidine and 5-methyl 2′-deoxycytidine were bought from Sigma and TCI.
Standard compounds for analysis were obtained from Sigma unless otherwise stated.
Optimized parameters for GC and GS were determined with individual standard compounds (Avanti Polar lipids, Inc).
In Table 1, concentration of standard compounds determined by HPLC in OS-EL is presented.
Chemical shifts were referenced to standard compounds under analogous conditions.
Quantitative analyses of standard compounds were performed on MRM data.
Diffraction patterns were interpreted by the PDF (ICDD) in-build library of standard compounds.
Identified peaks and corresponding standard compounds were indicated on the UPLC chromatogram.
Samples were separated by TLC as described above, but without staining of standard compounds.
As shown in Fig. 2, selected standard compounds were well separated by the developed HPLC method.
These observations were further verified using standard compounds dissolved in different proportions of ACN (Supporting Information Figure S-2).
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