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Whereas, at this staining period the less active protein bands were detected as clear white bands against dark background.
A 30-minute staining period was used.
Robust in situ hybridization signal in the nervous system was detectable after a short staining period.
The finding also explains why a long staining period was effective for these tissues.
After the staining period, cells are washed with culture medium and counted with a möLab cell counter (möLab, Langenfeld, Germany).
CT scans are serially taken throughout the staining period to monitor changes in contrast in distinct tissue types.
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The potential influence of the reaction of iodine with distinct tissues on the staining process over longer staining periods has not been discussed or quantitatively analyzed.
An improved understanding of both is essential for describing the staining mechanism and developing effective protocols, especially for larger specimens with longer staining periods.
Over the earlier staining periods (e.g., scans N-A-B), stagnation of the staining effect may be an indication of the sorption rate of GI complex is approximately equal to the rate of its decomposition or degradation.
Longer staining periods, such as overnight incubation (O'Rourke et al., 2009), saturated ORO staining in glp-1 ts) glp-1 tsto animals toat rendered glevelthatand glp-1(ts);skn-1 strendereddistinglp-1 tse. glp-1 ts
From the initial iodine concentration (around 1173 HU for CT value; equal to 2.97 % w/v for concentration), there was a significant drop of CT values in the solution for the first 3 6 days of staining consistent with previous results in which the best staining effect occurred in the earlier staining periods [ 9].
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