Sentence examples for Spindle checkpoint activation from inspiring English sources

Unexpectedly, we find that ipl1, spc24, and ndc10 -1 kinetochore mutants have a high level of PKA activity that is not due to spindle checkpoint activation or metaphase arrest.

Inactivation of rna14 leads to accumulation of Bub1-GFP foci, a protein required for spindle checkpoint activation that could be due to the defects in the attachment of mitotic spindle to the chromosome.

These studies showed that SW620 cells undergo synergistic cell death after spindle checkpoint activation followed by cisplatin treatment, suggesting a role of Chk1 in this checkpoint, very likely dependent on BubR1 protein.

There was a delay in Pds1 degradation in ctf8Δybp2Δ and ctf8Δ cells, indicating spindle checkpoint activation (Supplemental Figure S3B).

Several antimitotic drugs including the taxanes and the vinca alkaloids, via their effects on microtubules, prevent the formation of a normal mitotic spindle, resulting in spindle checkpoint activation.

Since in CLTC depleted cells MAD2 was found to be located at the kinetochores of both misaligned chromosomes and chromosomes at the metaphase plate, it was concluded that the persistent spindle checkpoint activation may be mediated by MAD2 signalling [5].

In these mutants, mitotic progression is halted attributable to spindle assembly checkpoint activation, and chromosomes remain in the vicinity of the spindle poles without congression.

For example, the checkpoint kinase Bub1, has a key function in recruitment of checkpoint proteins to kinetochores (Meraldi and Sorger, 2005) but also can phosphorylate Cdc20 to prevent it from interacting with APC/C or spindle assembly checkpoint components potentially acting to buffer Cdc20 levels during spindle assembly checkpoint activation (Tang et al, 2004).

The spindle checkpoint prevents activation of the anaphase-promoting complex (APC/C) until all chromosomes are correctly attached to the mitotic spindle.

MCC, which contains a second molecule of CDC20, blocks APC/CCDC20-UBE2C-dependent ubiquitination of Securin and Cyclins, while differentially determining or inhibiting CDC20 ubiquitination to regulate spindle surveillance, checkpoint activation, and checkpoint termination.

Detection of BubR1 indicates spindle assembly checkpoint activation.