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In vivo targeting was hK2-specific as verified by a 2.5-fold decrease in tumor uptake in pre-dosed xenografts or by a fourfold lower tumor accumulation in hK2-negative DU 145 xenografts.
The tumour accumulation of 111In-DTPA-11B6 was hK2-specific, as verified by the low accumulation in the negative control, DU145 xenografts and by competitive binding assays using excess cold non-labelled antibody.
Allele-specific Pyrosequencing verified 17 of them, including three novel imprinted genes.
Western blot analysis using a Pak1 specific antibody verified that all three groups of human Pak1 mRNA injected fish expressed similar levels of Pak1 protein (Fig. 5C).
Amplified products were resolved by electrophoresis using 10% polyacrylamide gels (Invitrogen) and specific amplification verified after ethidium bromide (0.5 µg/ml) fluorescence staining and imaging analysis (UVP Inc., Upland, CA).
Species-specific typing verified C. parvum in the Swedish isolates.
Information is selected by specific criteria, verified (if informal), and thoroughly analyzed before communication to the public.
The specificity of the phospho-specific antibodies was verified using Ala-substituted MYPT1 (see Figure 2A).
The specificity of the type-specific IFA was verified using plasma samples from the confirmed type I or II FCoV-infected cats or from SPF cats.
The neurons in the following tissue slice from the same region were also intensively stained with the NeuN neuron-specific antibodies, verifying that these are indeed neuronal cells (Fig. 3d).
None of the items in EDIS, however, appears as especially highly specific in verifying the presence or absence of infection, i.e. they do not solely correlate with infection or have sufficient sensitivity or specificity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com