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I don't think this came off as a chunk of foam and solidified with ice.
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*Medium without MS nutrient and sucrose but solidified with agar.
The medium was solidified with 3 g.
The media were solidified with 2% (w/v) bacto agar.
L plates were solidified with addition of 1.5 % agar.
Media were solidified with 20 g/L agar (ForMedium™).
For growth on plates, HMM was solidified with agarose and supplemented with 25 μM FeSO4.
Growth media were solidified with 15 g/liter agar (Difco) for the preparation of plates.
Transfer the whole mixture onto 90 mm Petri dishes containing PGM solidified with 1% agar.
Twitching motility was measured in LB solidified with 1.2 % bacto-agar.
The gelatin-embedded cells were solidified on ice, and then incubated with PVP-sucrose cryoprotectant (20% polyvinylpyrrolidone, 1.84 M sucrose, 0.055 M Na2CO3, pH 7.4) for 24 h at 4°C.
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