Since transcript accumulation of PtTPS15 was strongly upregulated after herbivore damage, it is likely that PtTPS15 contributes to the production of the precursor for DMNT biosynthesis in planta.
Since transcript phase is a circular dependent variable (i.e., phase CT24 is equal to CT0), we needed a link function to relate the linear predictor based on the three scores to the transcript phase.
Since transcript isoforms could be derived from orthologous genes from closely related bacterial species or strains, they were not collapsed to the gene level prior to in silico functional annotation.
Since transcript abundance was measured using two different techniques: qRT-PCR and microarray that might lead to a non-linear relationship, Spearman's rank correlation coefficients, which are free from parametric assumptions, were used to compare the outcome of these two techniques.
Since transcript levels for genes involved in iron homeostasis were altered, suggesting a shortage of biologically available iron induced by CORM-3 stress, we measured intracellular iron levels in wild-type and hemA cells under the same conditions used for microarray experiments.
Furthermore –again similar to Arabidopsis roots– increased PSY seems sufficient to explain heightened carotenoid accumulation, since transcript levels of other carotenogenic genes do not increase in orange as opposed to white-rooted carrots [32].
Moreover, Zambelli et al. suggest that the orthology relationship should be considered at the transcript level, since transcript isoforms are the individuals subject to protein functions [ 13].
Furthermore, since transcript levels of AtPNP-A were reduced beyond detection limits in senescing leaves in the nahG mutant this induction appears to be SA-dependent.
