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Similar assay conditions were used to determine equilibrium points.
In a similar assay, we tested the antifungal activity of MCETs by degrading their DNA backbone with DNase.
Under similar assay conditions, the contaminated activities of arylsulfatase in crude extracts were also determined.
These EAs can be measured in air-dried soil with appropriate substrates following similar assay conditions.
In contrast, under similar assay condition, PARP1 alone could not add branched PAR chains on the existed PAR chains (Fig. 2c).
To further corroborate that mutated GLUT5 in yeast behaves like the WT protein in animal cells, we performed a similar assay with ECG, an inhibitor that is structurally not related to MSNBA.
For example, Enzalutamide has been reported to bind AR with 5 8 fold greater affinity than Bicalutamide using a radiolabelled DHT displacement assay27, with a similar assay suggesting ARN-509 bound with 7 10 fold greater affinity than Bicalutamide45.
This method should be useful for engineering of the xylose transport process in E. coli, and similar assay schemes can be extended to other microorganisms.
The assay sensitivity on the proposed platform was 3-orders of magnitude lower to the similar assay executed on pristine filter paper.
A similar assay using t-BBN/BVD15-DO3A against porcine 125I-NPY showed IC50 values of 80 ± 11 nM for Y1 receptor in MCF7 cells, another human breast cancer cell line.
The nested-PCR standardized in this study (nPCR-SH) was compared in terms of sensitivity with another similar assay (nPCR-XIAO) that has been largely used for the detection and identification of Cryptosporidium spp. worldwide.
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