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Both amplifiers on mfERG1 were set to amplify 100,000 times.
Different from conventional approaches, two dedicated designed thresholds are set to amplify the small signals and compress the large signals, respectively.
Sperm DNA was extracted and PCR analysis was done using one primer set to amplify a single copy section of the factor IX precursor (X-chromosome only) and another primer set to amplify a single copy section the sex determining region (Y-chromosome only).
The other PCR was intended to screen for annelid DNA and used an annelid-specific primer set to amplify a 416 bp fragment of the annelid 28S ribosomal RNA.
We designed a primer set to amplify part of the mitochondrial cyt b gene of both species and obtained 1024 base pairs (bp) of polymerase chain reaction (PCR) product.
In this study, we designed a new primer set to amplify a 668 bp-region within the RNA-dependent RNA polymerase (RdRP) gene that encodes the most conserved protein domain of α-, β-, γ-, and δ-CoVs.
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However, high sequence variability of the viral agents posts a challenge to the design of robust universal virus-specific primer sets to amplify various virus variants.
For Rh genes, we designed 10 primer sets to amplify all 10 exons.
We therefore designed three new primer sets to amplify sequences that contained just a single microsatellite-containing insert and these were found to amplify successfully.
Additional file 4: Primer sets to amplify of target transcripts.
We chose primer sets to amplify the three a, b, and c full-length isoforms (http://www.ncbi.nlm.nih.gov/ieb/research/acembly/).nih.gov/ieb/research/acembly/
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