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An over-expression E. coli strain was constructed by transforming E. coli ER3081 (NEB) with pSYX20-hpyAVM and pAII17-hpyAVR by sequential transformation (See Materials and Methods).
The simplest sequential transformation is retiming.
The past several decades have seen dramatic progress in understanding of pancreatic cancer pathogenesis, including identification of precursor lesions, sequential transformation from normal pancreatic tissue to invasive pancreatic cancer and corresponding signature genetic events, and the biological impact of these events on malignant behavior.
Indeed, the bulk for the oxygen requirement centers on the sequential transformation of lanosterol to cholesterol, involving several redox reactions.
AES consists of 40 sequential transformation steps composed of simple and basic operations such as table lookups, shifts, and XORs.
This idea, also known as the retrograde hypothesis, has intuitive appeal and states that if the contemporary biosynthesis of compound "A" requires the sequential transformation of precursors "D," "C," and "B" through the corresponding enzymes, the final product "A" of a given metabolic route was the first compound used by the primordial heterotrophs (Fig. 8).
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Nevertheless, in order to saturate screening, it is advisable to perform sequential transformations which mostly results in a few million transformants.
Several families of compounds are synthesized by sequential transformations.
More complex sequential transformations modify both the logic structure and the positions of the latches.
The α- and δ-relaxation times were found to be sensitive to the sequential transformations between mesophases.
A special place among sequential transformations is given to integrated sequential optimization, which can achieve the globally optimal delay of the circuit by performing a sequence of simple local transformations, such as local restructuring and retiming individual nodes.
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