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Sequential filtering results in blue-shifting of the NIR peak and removal of the larger nanoplates.
For the bare-earth extraction, we used hierarchical robust filtering algorithm and a parameterization with three sequential filtering steps.
To define genes deregulated by TGF-β signaling, three sequential filtering steps were applied for each time point separately: 1) significant difference between TGF-β stimulated and unstimulated cells, 2) significant deregulation by at least one compound concentration, 3) dose dependent deregulation (R package IsoGene [51]).
POTION computes a fourth optional sequential filtering step where users can define an anchor genome and therefore analyze only groups that, after all the previous sequence filtering steps, contain at least one gene from the anchor genome.
Since our sequential filtering strategy favours-specificity at potentially great cost in sensitivity, we also undertook a pooled analysis of our human data and the publicly available results of Sabates-Bellver et al. (4) who used an Affymetrix platform which was compatible with our analysis.
Results show steady-state sequential filtering to be effective and robust.
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The application of sequential filters (drug-likeness, 3D-pharmacophore mapping, docking, molecular dynamics simulations) yielded a hit list of compounds, out of which 9 were tested in the in vitro AlphaScreen assays and 8 exhibited a detectable inhibition of the interaction between the two proteins.
We define also the homotopic alternating sequential filters [5].
We introduced also the homotopic alternating sequential filter which constitutes base for topological smoothing.
The homotopic alternating sequential filter is a composition of homotopic cuttings and fillings by balls of increasing radius.
Segmentation region denoising can be achieved via the use of an alternating sequential filter (ASF) as given in [73].
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