Sentence examples for Sequence verification from inspiring English sources

Sequence verification was performed using a CEQ capillary sequencer (Beckman Coulter, Fullerton, CA).

Sequence verification of PCR-generated fragments was performed by sequencing.

After sequence verification, the plasmid was digested with restriction enzymes EcoRI and HindIII to isolate the DNA fragment containing the mutated NT* sequence.

A cDNA clone containing the coding sequence of the rat cannabinoid receptor type 1 (CB1) (I.M.A.G.E. expressed sequence tag clone, GI Accession #11375084) was linearized after sequence verification.

The ligation mixture was heat-shock transformed into chemically competent E. coli Nova Blue cells followed by plasmid preparation and sequence verification.

High Resolution Melting (HRM), amplicon size quantitation and sequence verification is used to screen for non-target amplification (false positives).

Following sequence verification, the fragments were cloned into the pCAMBIA1301 binary vector (CAMBIA; www.cambia.org.au).au

After sequence verification and LguI/Eco81I double digestion, genes were ligated into LguI-linearized pQE-30-LE to give plasmid pQE-30-LE::bgl1 (Eurofins, Ebersberg, Germany).

After sequence verification, the HvNAS1-tNOS fragment was digested with SpeI, whereas the OsActin1 promoter in the SK vector was digested with XbaI.

The amplicon was ligated into p426GPD (Mumberg et al. [1995]) resulting in p426GPD-YAP1 used for transformation of E. coli DH5α cells and followed by sequence verification.

Mouse PLD2 cDNA was similarly cloned by RT-PCR and was used following sequence verification.