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All sequence read processing and plastome assembly were performed using Geneious v.6-7 (BInc.tters, Inc., Auckland, New Zealand).

Table 1 Basic statistics derived from read processing and mapping.

Particular importance was placed on the analysis options available for this type of data and consideration was given to sequence read processing, bovine genome assemblies available for alignment, and mapping strategy.

Sequencing reads processing (adapter trimming with AdapterRemoval v2.2.273), mapping (read alignment, PCR duplicate removal, and indel realignment), and damage analyses (mapDamage v2.0.674) were performed using the PALEOMIX v1.2.13.1 pipeline75.

Sequence reads are counted and mapped to a reference transcriptome.

CONSED [ 134] was used for sequence manipulation that included read/contig editing, primer design, and finish read processing.

Sequence reads were mapped and annotated using Bowtie2 software [ 43].

Sequence reads were mapped to the reference sequences NC_003030 (chromosome) and NC_001988 (pSOL1) using CLC Genomics Workbench.

Above 75% of the bisulfite sequence reads were uniquely mapped and produced meaningful methylation data.

In our study, sequence reads were mapped to the UCSC hg19 reference genome using Bowtie2 and TopHat.

The statistics for read pre-processing and mapping were displayed in Table  1.

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