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To maximize the sensitivity of quantification, we manually cut out a 0.3 cm-wide strip containing the surface biofilms formed at the air-liquid interface.
In order to provide an empirical estimate of the sensitivity of quantification, two fluorescently labelled transferrin probes were mixed and added to the cells.
The sensitivity of quantification in expression studies to different biases underlines the importance of validating expression levels of candidate genes using a great number of biological and also technical replicates.
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The proposed methods showed good sensitivity, limits of quantification were lower than MRLs and precision (expressed as relative standard deviation) ranged from 2.9 to 11.1%.
The selectivity, sensitivity (limits of quantification were in the range of 0.02 11.0 ng g−1), accuracy (recoveries ≥77%) and precision (RSD ≤ 30%) obtained were quite adequate for the determination of very low target PAHs in sediment samples.
The developed method provides a good linearity (R2 = 0.9986) and sensitivity (limit of quantification of 10 μg/L), especially if the low sample volume (200 μL) used is considered.
During photo-diode array/fluorescence detector method validation, satisfactory analytical characteristics, in terms of selectivity, recovery (72.7% 109.1%), precision (inter-day RSDs <9.6%), sensitivity (limits of quantification ranged from 2.1 μg kg−1 to 120.0 μg kg−1) and linearity (R2 all higher than 0.9984), were achieved.
This was the first report of PCR-based quantification of sequencing libraries, and extended the sensitivity of library quantification significantly – although to an unknown extent, since the source material used to make the Neandertal (presumably the lowest input quantity) libraries was not quantified.
The results show that the combination of multi-wall carbon nanotubes and a modifier causes a dramatic enhancement in the sensitivity of FA quantification.
Moreover, assessing protein levels of cytokines in the brain is hampered by the limited sensitivity of available quantification techniques.
The human genome accommodates multiple AluYb8 repeats, which explains the high sensitivity of the quantification assay used here.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com