Sentence examples for Secondary assay from inspiring English sources

The method required two sequential polymerase chain reaction stages, a primary and secondary assay.

The primary unlabeled probe data limited the possible genotypes for the detected RET sequence variation, which permitted genotyping in a secondary assay with only two to five reactions.

Of the 60 hits selected for follow-up studies, 14 displayed IC50 values below 20 μM under the secondary assay conditions, and 4 showed an activity in cellular tests.

1218 (P) -0.11 -0.16 -0.13 Functional assay for estrogen-mediated translocation of PIP3: Secondary Assay for Estrogen receptor beta.

1219 (P) -0.11 -0.16 -0.13 Functional assay to assess estrogen-mediated translocation of PIP3: Secondary Assay for GPR30 estrogen receptor.

When the primary assay hit, but the secondary assay was not performed, the Ki value is also treated as >10,000.

In combination with a variety of secondary assays, these results provide insights into the mechanism and dynamics of somatic pairing.

Some of the assays discussed have been utilized in antiviral screens while others might be formatted for HTS or utilized as secondary assays in a screening campaign.

Finally, secondary assays built into the cell system can differentiate between modulators that stimulate, inhibit, or do not affect the transcriptional activity of the receptor under study.

A commitment of resources to transfer the biological assay to HTS format, run the screen, confirm statistically significant hits, analyze the results, and follow up with secondary assays must be in place.

We focus on one such compound, BRD6897, and demonstrate through secondary assays that it increases the cellular content of mitochondria as evidenced by fluorescence microscopy, mitochondrial protein content, and respiration, even after rigorous correction for cell size, cell volume, or total protein content.