Sanger sequencing method (costs ∼$130 per mtDNA) and mtDNA data analyses are described in a separate report (unpublished data).
Sanger sequencing method for 4G/5G genotype detection is relatively rarely used by other authors [ 26].
Sanger sequencing method was used to obtain targeted BAC sequencing data as previously described (Hamberger et al., 2009; Keeling et al., 2010).
To determine rate of false positives in our set, we randomly selected 30 SBSs (Additional file 2: Table S1A), designed primers flanking the predicted substitution sites, amplified the fragments and re-sequenced using Sanger sequencing method at Genewiz , Inc
DNA isolated from specific splice isoforms was sequenced by Sanger sequencing method.
From such screening, 356 clones were found positive and were subjected to sequencing using Sanger sequencing method.
The pyrosequencing technique was found to be superior to the microscopy method and more rapid than the Sanger sequencing method.
The clinical sensitivity and specificity of the assay was compared to the gold standard Sanger sequencing method using 25 sensitive, 15 resistant and 20 negative samples.
A proportion of samples was further typed by Sanger sequencing method to obtain higher resolution for the HLA type (Commercial AlleleSEQR kits 08K60-06, 08K62-06, 08K62-06, 08K63-06, GenDx, Utrecht, Netherlands).
Researchers use the two robots, Tecan Genesis and Beckman Biomek FX to process DNA sequencing samples, using the Sanger sequencing method and next-generation genotyping for large-scale plant and animal breeding programs.
Initially, many miRNAs in rice were sequenced through the traditional Sanger sequencing method, most of which turned out to be the high-abundance miRNAs [16, 33].
