However, the expression of CDC2 was both negative in brain tumor stem cell spheres and neural stem cell spheres.
Expression of CDC2/CyclinB1 was very high in SHG44 and GBM, but was negative in brain tumor stem cell spheres and neural stem cell spheres.
Although the endogenous MIR520G locus is methylated in differentiated cells, exposure of DAOY cells to 5-aza-2′-deoxycytidine or their growth as stem cell like spheres up-regulated endogenous miR-520g with a coincident reduction in TF expression.
Brain tissue from a 1-month-old abortive dead embryo was subjected to the same procedure as described above to get neural stem cell spheres [ 6].
Under the culture conditions identical to those for neural stem cells, spheres resembling typical neurospheres readily formed on top of the monolayers of both cells.
In other reports, Gata-4 expression has been described in some neural crest-derived cardiac progenitors (Tomita et al, 2005) and in primitive neural stem cell spheres (Hitoshi et al, 2004).
Our induced cancer stem-like sphere cells, SK-spheres and HLE-spheres, showed lower susceptibility to 5-FU, cisplatin, carboplatin, doxorubicin, docetaxel, SAHA, and sunitinib than their parental cells.
In contrast, when cells were cultured under stem cell (sphere) conditions, no disaggregation became apparent upon integrin inhibition, and cell death was not observed.
In our condition, numerous cancer stem-like sphere cells were obtained easily.
PEITC attenuated proliferation of CD44high/+/CD24low/–, stem-like, sphere-forming subpopulations of hCSCs in a concentration- and time-dependent manner that was comparable to the CSC antagonist salinomycin.
