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SP cells were not detected in control (Fig. 5A).
SP cells from HNSCC were highly tumorigenic, invasive and chemoresistant.
SP cells in HNSCC were highly invasive in vitro and tumorigenic in vivo compared to non-SP cells.
SP cells also demonstrated much higher colony formation ability compared with non-SP cells.
SP cells resisted chemotherapy compared with non-SP cells, both in vivo and in vitro.
SP cells were hardly detected in the other cell lines.
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FLK1 single positive (F-SP) cells contain lateral plate mesoderm with haematopoietic/endothelial potential and PDGFRα single positive (P-SP) cells contain paraxial and cardiac potential.
The expression of chinmo indeed increased in ph505; UAS-let-7-SP cells (Supplementary Fig. 6h), but did not in ph505; UAS-miR-125-SP cells (Supplementary Fig. 6i).
Furthermore, when we transplanted adult ph505; UAS-miR-125-SP cells into host flies (Supplementary Fig. 6f), they did not give rise to neoplastic tumours, indicating the ph505; UAS-miR-125-SP cells are not tumorigenic anymore.
Indeed, when compared to day 3.5 Scl−/− F-SP cells, a greatly decreased number of day 4.5 Scl−/− F-SP cells acquired low level PDGFRα expression in the re-aggregation assay (Fig. 2c, right panels).
We observed that ph505; UAS-let-7-SP cells maintained the tumorigenic growth in the adults (Fig. 3b).
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