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The 4 position signals (Xa, Xb, Yc and Yd) were digitized using a free running sampling technique.
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BD fluorosphere counting beads were added to cocktails before running samples.
Running sampler faster, or having less samples, in contrast, makes sampler less stable, and unwanted false move would happen.
The specificity of this monoclonal antibody was confirmed by running samples under reducing conditions.
Extracted RNA was quantitated by spectrophotometry and quality evaluated by running samples on denaturing agarose gels.
Genomic quantification was carried out by running samples of the standard curve with unknown samples.
The column was equilibrated and cleaned with 5% CH3CN for 10 min between running samples.
The integrity of RNA was determined by running samples on a 1.3% agarose gel.
Calibration was done by running samples with known concentrations of glucose and maltotetraose.
Transcript size was determined by running samples on an Agilent 2100 BioAnalyzer.
PCR amplification was verified by running samples out on a 1% agarose gel.
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