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The completion of the Human Genome Project, together with advances in sequencing technologies have directed the emergence of a panel of revolutionary sequencing technologies termed next-generation sequencing (NGS).
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RNA sequencing has been one of the first applications of the revolutionary ultrahighthroughput DNA sequencing technologies [ 1- 9].
Remarkably, the cost of genome sequencing has been drastically reduced in the last two decades due to the revolutionary advancement of DNA sequencing technologies.
Prenatal testing is a burgeoning industry for DNA sequencing technologies (see "DNA Sequencing Leader Buys into Prenatal Testing" and "Prenatal DNA Sequencing").
Such studies would optimally employ RNA sequencing technologies, and would be expected to identify a larger number of exosome-expressed miRNA.
The Yale study used powerful DNA sequencing technologies to examine 147 melanomas originating from both sun-exposed and sun-shielded sites.
Recent advances in RNA sequencing technologies have greatly expanded our knowledge of the RNA landscape in cells, often with spatiotemporal resolution.
Faster and more cost-effective DNA sequencing technologies are accelerating investigations of a broad range of genomes – from model organisms to pathogens that may have a direct impact on human health.
This approach, particularly when combined with new RNA sequencing technologies, should also be useful in further defining Pol II transcripts differentially regulated in specific disease states and in studying RNAs regulated by changes in pre-mRNA splicing or 3' polyadenylation status.
RNA sequencing technologies reveal that bacteria express RNA molecules other than mRNA, rRNA or tRNA.
But RNA sequencing technologies do not always represent the most appropriate strategy for large scale transcriptomics.
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