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Surgeons should review the specimen imaging before the operation has been completed, ideally with surgeon-radiology communication.
Occasionally, if surprised by a low LN count, surgeons might ask the pathologist to take another look (537) or they might review the specimen with the pathologist (547).
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In case of disagreement, both pathologists reviewed the specimen under a double-headed microscope and reached a consensus diagnosis.
As preoperative chemoradiation affects nodal harvest, the number of nodes that should be examined following preoperative treatment is not defined, but 15 nodes should be sought out by the surgeon and pathologist reviewing the specimen.
Perforation was considered to be present if noted by the surgeon at operation or on histopathological review of the specimen.
In analyses of tumour behaviour and histologic subtype, classification was improved by having the study pathologist abstract data from all of the pathology reports; however, without a formal review of the specimen slides it is possible that there may be some misclassification.
A histological classification for each microdissected target was assigned based on review of the specimens and accompanying histopathology reports from the original microscopic review.
Three uropathologists review the pathology specimens and classify each according to the modified Gleason grading system proposed by the International Society of Urological Pathology (ISUP) [ 10].
The authors thank Dr. Preetha Ramalingam for help with Pathology Review of the Specimens, and Vasanta Putluri for technical assistance.
JS performed the histopathological review of the specimens.
MK performed the histologic review of the specimens.
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