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3. a need for more extensive reproducibility testing with the use of more paired CSF and serum samples analyzed at least twice in different runs.
Reproducibility testing verified that no differences in PFGE patterns were observed between experiments.
First, design, optimization and reproducibility testing of this custom-built device were carried out.
Two other points closely connected to method validation are also examined: incurred sample reproducibility testing and measurement uncertainty as they are highly linked to bioanalytical results reliability.
Every step has to be understood and extremely well documented and capable of being done by anyone". In addition, he says, the safety and reproducibility testing required by the FDA is much more rigorous than that needed for pilot clinical trials.
After completing reliability and reproducibility testing, which showed a margin of error of 7%, 10 volunteers were measure for quantitative electromyographic activity at the L5 paraspinal level in full lumbar flexion without the Disk Unloader brace 3 times and the results were averaged.
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Finally, interinstrument reproducibility, tested by means of the same protocol on three different instruments, all operating at the same magnetic field and using largely identical hardware for each location, was found to be 1-14% (T1) and 2-10% (T2).
Intraobserver reproducibility, tested for 10% of randomly selected images (213/2,130), was 0.93 (Pearson correlation coefficient).
Within-day reproducibility was explored using a one-hour break between sessions, and between-day reproducibility tested with 1 7 days between sessions (Fig. 1).
Reproducibility tested the null hypothesis that the mean difference between the coefficients of variation (CVs) of observations was zero using a paired Student's t test.
For the reproducibility test, 30 individual cells were fabricated with the PCE distribution histogram shown in Fig. 7d and detailed parameters are summarized in Supplementary Table 8, both confirming very good reproducibility.
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