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Red: dextran.
Texas Red dextran (70 kDa, Molecular Probes) was administered intravenously by retro-orbital or tail vein injection.
Intravital TPLSM recordings performed 3 min (first part of video) and 30 min (second part of video) after i.v. injection of 70 kD Texas Red dextran dye 3.5 days p.t. of TbSYN cells.
For the Texas Red dextran experiments (10.000 MW, 10 µg/mL-Invitrogen, Molecular Probes, Inc., Carlsbad, CA), peritoneal macrophages were treated with Alexa Fluor 488-labeled pcDNA3-Hsp65 (4 µg) in growth medium containing Texas Red dextran for 24 hours.
Unsynchronized NRK cells in metaphase were injected with G2A GRASproteintoinhibitnhibit stacking, together with the CD8 plasmid and Texas Red dextran as a marker.
After the cells were incubated with fluorescent DNA plus Texas Red dextran, they were washed twice with PBS, fixed for 15 minutes with 2% paraformaldehyde in PBS, rinsed in PBS and coverslips mounted with Fluormount (EM Sciences, Hatfield, PA).
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To analyze the kinetics of CD8 transport during interphase, NRK cells were microinjected with 0.2 mg/ml pcDNA CD8 and 2 mg/ml Texas-Red dextran as a marker.
MO was injected with Texas-Red Dextran (Invitrogen). 1 mM MO was used for microinjection.
Red, Texas red dextran-labelled vessels, macrophages.
METHODS: PLGA nanoparticles were fabricated using a double emulsion method and were loaded with Texas Red-dextran (NPTR), hepatocyte growth factor (NPHGF), and bovine serum albumin (NPBSA).
In brief, 1 2 cell stage Gateways transgenic embryos were injected with Texas Red-dextran.
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