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The cleaned reads of each species library assembled by ABySS and SOAP denovo produced 24 and 25 raw assemblies, respectively (Additional file 6).
"River Cottage Veg" comes to the rescue with recipes for salads, pasta and rice dishes, meze and tapas dishes, "raw assemblies" like radishes with butter and salt, and "bready things" like beet pizza with Cheddar.
Toasted seeds and nuts are fabulous additions to raw assemblies, while ready-made, pre-cooked and even tinned foods are often found alongside raw veg and fruit in my salads, picnics and lunchboxes: breads and cheeses, lentils and beans, cooked and cured meats, all mingle well with the uncooked.
The final assembly also had a higher proportion of annotated transcripts (~40%) than all raw assemblies.
Sources of DNA sequences corresponding to raw assemblies of basidiomycete genomes are listed in table 1.
Twenty-five rassembliesies from each species library were further merged using the same methods described above.
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Table 1 Assembly statistics information Raw assembly Duplicate removed assembly Trinity 'genes' 27,389 24,000 Trinity transcripts 31,068 25,234 GC content 40.84 40.71 Median contig length (bp) 722 622 Average contig length (bp) 1269.6 1160.9 Total assembled bases (bp) 39,444,068 29,294,166.
The assembly was carried out using Cufflinks [ 25] to generate two kinds of assemblies; raw assembly and novel assembly.
From the raw assembly from group 5 new annotation for the PTENP1 asRNA was constucted.
The raw assembly is available at http://athyra.ged.msu.edu/~preeyano/seal/Mirounga_raw.fa.gz.msu.edu/~preeyano/seal/Mirounga_raw.fa.gz
For the raw assembly all mapped reads were used and no reference annotation was used to guide the assembly.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com