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Biopsy specimens for RNA purification were immersed in RNA later RNA stabilization reagent (Qiagen) and stored at 4°C overnight and thereafter at −20°C until RNA purification.
The biopsy specimens used for RNA purification were immersed in RNA later RNA stabilization reagent (Qiagen, Hilden, Germany) and stored at 4°C overnight and at −20°C thereafter, awaiting RNA purification.
Brains and spleens were excised and immediately submerged in RNA later® RNA stabilization reagent (QIAGEN, Hilden, Germany) [ 23].
Birds were sacrificed through decapitation and the entire brain was immediately transferred into a tube containing RNA later™ RNA stabilization Reagent (Qiagen, cat. no: 76106).
Each sample of excised gastric tissue was immediately immersed in RNAlater RNA stabilization Reagent (QIAGEN Sciences, Maryland, MD), and the samples were homogenized for 60 sec at 2400 g using a MagNA Lyser (Roche diagnostics Inc., Mannheim, Germany).
After treatment, the five to six OC explants were pooled together as one biological sample and stored in RNA stabilization reagent (QIAGEN, Hombrechtikon, Switzerland) at −20 °C until further use.
Similar(38)
Prior to GEP MMPC need to be enriched resulting in an inability to immediately freeze bone marrow aspirates or use RNA stabilization reagents.
The instability of bacterial mRNA can in part be overcome by using commercially available RNA stabilization reagents such as RNAlater (Qiagen, Hilden, Germany).
Importantly, prior to expression analyses MMPCs need to be enriched preventing the immediate freezing of bone marrow aspirates or the use of RNA stabilization reagents after removal from the patient.
The collected tissue samples were rapidly embedded in RNALater RNA Stabilizations Reagent (QIAGEN, Hilden, Germany) for further molecular analysis.
The sample was treated with RNA later and stored at −80°C until analysis, at which point it was centrifuged to remove the RNA later stabilization reagent.
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