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Exact(56)
The fluorescence signal magnitude increases as rcap1.8 where rcap is the capillary radius.
where rspot is the primary spot radius measured at 1/e, rcap is the capillary radius and WD is the detection capillary working distance.
Since the sides of the rectangles ({R_{i}}) parallel to the (x_{2}) direction are in decreasing order, it is easy to obtain Rcap biggl[bigcup_{ileq k,Rcap R_{i}^{ast}neq emptyset}hat{R}_{i}^{ast} biggr]= biggl Icap biggl[bigcup_{ileq k,Rcap R_{i}^{ast}neqemptyset} hat{I}_{i}^{ast} biggr] biggr times J, where (hat{R}_{i}^{ast}=hat{I}_{i}^{ast}timeshat{J}_{i}^{ast}).
Figure 6 shows the network length and the network growth rate as functions of the ADF/cofilin binding rate rac and the capping rate rcap.
To further improve the purity of the rCap, the GST-column purified rCap was subjected to cation exchange chromatography.
However, rCap protein when used against some pigeon sera did not show very strong antigenicity.
All above fusion tags were fused with recombinant rCap at its N-terminus.
The expression patterns for the rCap fusion protein were relatively poor in BL21 DE3 -pLysS.
Similar(3)
No matter whether the His-rCap or GST-rCap protein was being expressed, the protein products were almost undetectable.
Overall, expression level of the Trx-His-rCap protein, was significant higher than that of the GST-rCap protein in BL21 DE3 -RIPL strain.
Proteins at the predicted molecular weights, 32 kDa His-rCap, 58 kDa GST-rCap and 48 kDa Trx-His-rCap, were not detected using anti-His monoclonal antibody and anti-GST antibody as appropriate.
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