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Extracted DNA was isolated using a modified Qiagen MinElute silica spin column purification protocol.
A common protein purification protocol was utilized for all KpBest and bBest2 proteins in this study32.
Overall, the purification protocol yielded high level of purity (>90%).
The quartz phase was removed by applying a purification protocol based on sedimentation.
Isolation of this aggregated material, combined with specific washing procedures, was the most significant stage of the purification protocol.
(C) Cell counts of cultures confirmed that 90% of cells are labelled as motor neurons after the purification protocol.
We developed a purification method from an ethanolic extract by using a diatomite-based purification protocol.
The figure describes each step in the purification protocol and further explains the denotations P1, S1, P2, S2 and T in Fig. 4e,f.
RNA isolation was performed using the mirVana Kit (Ambion, Thermo Fisher Scientific GmbH, Schwerte, Germany) following the manufacturer's total RNA purification protocol.
Cells infected by either recombinant virus can be sorted differentially by a simple and reliable FACS-based purification protocol.
The optimized purification protocol in the presence of dodecyl β-d-maltoside allowed isolation of highly homogeneous MexB.
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