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According to Protparam software (http://www.expasy.ch/tools/protparam.html) the estimated half-life for FOXL2 in mammalian reticulocytes is around 30 hours, and the instability index is computed to be 66.04, classifying the protein as unstable.
Properties were calculated by means of the online ProtParam software, http://us.expasy.org/tools/protparam.html[ 80].
The molecular weight for each GmCYP was calculated using ProtParam software [ 47] (http://web.expasy.org/protparam/).org/protparam/
The biochemical properties of putative uterine proteins (pI and amino acid composition) were determined with Protparam software [ 83] VJ was involved in designing and planning of the study.
The concentration of purified CBM was determined spectrophotometrically from absorbance measurements at 280 nm using extinction coefficients individually calculated for each CBM using the ProtParam software [ 16] available at http://www.expasy.org.org
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The Expasy software Protparam [25] was used to achieve this.
Briefly, Protparam [25] is a proprietary computational software available at Swiss Prot/UniProt that can be used to derive series biophysical profiles as well as determine the amino acid composition of any protein or polypeptide of interest, provided its primary structure (linear alignment of amino acids) is known.
For putative proteins ExPASy-Prosite [ 13], ExPASy-ProtParam [ 14], pSORT [ 15], and SMART [ 16], software analyses were performed.
Calculated masses were obtained using the ExPasy ProtParam tool (http://web.expasy.org/protparam/).org/protparam/
Expasy ProtParam classified this chimeric protein as a stable protein.
The protein concentration was measured by spectrophotometry at λ = 280 nm, with an extinction coefficient of ScASNase1 (ε=25955 M−1 cm−1) obtained using the ProtParam tool (http://web.expasy.org/protparam/).org/protparam/
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