All of the examples mentioned above are based on the single-cell patch, but the most problematic issue for realization of a high-throughput system is the reproducibility of the electrical sealing.
In particular, the assumption of independent realizations can be problematic for spatial or temporal data, although in principle, adjustments for such difficulties sometimes can be made.
So far, the realization of SAPV is very problematic; e.g., there are no uniform standards regarding the staffing and structural requirements of palliative care teams, and their cooperation with other players (especially family doctors, nursing services, specialist physicians in other medical disciplines) is not consistently regulated [ 11].
Even then, several cautioned that the broader impact of financial deficits and tightening of NHS funding streams, combined with the knock-on effects of increased pay bill costs as Agenda for Change was implemented fully, meant that full benefits realization would be challenging and problematic.
Curiously, while the logic \(\mathsf{S4LP}\) seems quite natural, a Realization Theorem has been problematic for it: no such theorem can be proved if one insists on what are called normal realizations (Kuznets 2010).
One might hope that children who had this experience – a realization that their diabetes was more problematic than they had originally perceived – would make healthy behavior changes such as testing blood sugar more frequently and come back to camp the next year in tighter glycemic control.
As the possibility of a clear division between domestic and foreign affairs dissipates, the traditional tendency to picture the domestic arena as a privileged site for the realization of normative ideals and principles becomes problematic as well.
The idea of private donors footing the legal bills for government officials is inherently problematic, although any outrage should be tempered by the realization that even a low-level employee who is simply called as a witness can easily rack up tens of thousands of dollars in personal legal fees.
Later in the piece, Lowry adds, "Bush's foreign policy has obviously needed adjustments: more of an emphasis on diplomacy and allies; a realization that creating democracy through military intervention is deeply problematic; a greater measure of prudence".
That plants and trees or the land itself have a similar potential for spiritual liberation became an explicit doctrine in Chinese and Japanese Buddhism but may even have been part of popular Buddhist belief from earliest times in sum, a realization that all life-forms share both a common problematic and promise.
The second problematic feature of deep ecology concerns the principle of self-realization, which claims that the human self (small 's') is actualized only when it becomes merged with the cosmos, a Self (capital 'S').
