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Prior to loading sample into the microscopic chamber, the IR source and interferometer were evacuated below 100 Pa.
Prior to loading on the sequencing gel, the above mixtures were heated at 65 °C for 2 min, prior to loading 2 μL of each samples.
Overall, 30 samples were collected at the beginning of the trials prior to loading the logs onto the pallets.
The lysate was syringe filtered prior to loading the Strep-Tactin column.
CM was normalized to the cell number prior to loading the gel.
The columns were conditioned with 12 mL of n-hexane prior to loading the sample.
If using a licensed boat transport you should do an inspection of their equipment prior to loading your vessel.
Prior to loading, the cannon would be well cleaned with a sponge to remove all sparks, filth, and dirt.
Prior to loading, the biological sample was placed in a solution of 50% H2O, 50% glycerol and 100 mM NaCl.
Prior to loading, the samples were diluted ten times with water.
Prior to loading, samples were heated to 80°C, vortexed, and centrifuged.
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