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Prior to further processing, solutions resulting from either acidic or carbonate leaching must be clarified.
Prior to further processing, all of these signals were then full-wave rectified.
Prior to further processing, RNA quality was ascertained by electropherograms on the Agilent 2100 Bioanalyzer.
Prior to further processing, the raw read data were subjected to the MG-RAST online server [ 27] to remove duplicate and low quality reads.
Prior to further processing, sections were allowed to thaw completely and fixed in methanol-acetone (1 : 1) for 10 min at −20°C.
Prior to further processing, the raw read data were subjected to the Metagenome Rapid Annotation using Subsystem Technology (MG-RAST) v.3.0 online server quality control pipeline [ 26] to remove duplicate and low quality reads (Additional file 1).
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New methods are currently being developed to automatically detect channels with poor signal quality, allowing them to be deleted prior to further data processing [ 32].
Briefly, animals were anesthetized in ice, and the sinus glands were dissected and collected in chilled acidified methanol and stored in −80 °C freezer prior to further sample processing.
Since IVD cell matrix degeneration predominantly occurs in cells producing collagen type II, all specimens were verified to be collagen type II positive by immunhistological staining analyses prior to further analytical processing (data not shown).
A small number of false SNPs can be easily detected during post-processing analysis of genotyping calls and filtered out prior to further analysis.
dsRNA products were stored at −80 °C prior to further use.
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