Sentence examples for Primary labeling from inspiring English sources
Primary labeling was performed using chicken anti-c-myc epitope tag (1 1000) antibody and single concentration (2 μM) of soluble proteins.
Primary labeling of AcTub and HA was performed with mouse anti-AcTub (1 1000; Sigma) or rat anti-HA (1 1000; Roche Applied Science), respectively, for 1 hr.
Most commercially available antibodies are available as a FITC or carboxyfluorescein-labeled conjugate in order to use primary labeling instead of secondary labeling.
After the primary labeling, cells were washed three times with PBS-1% saponin, and incubated with fluorescent secondary antibodies in absence of light for 1 h.
This primary labeling of UBIAD1 protein was then visualized by incubating keratocytes with 5 µg/ml Alexa 594 (red) streptavidin diluted in DPBS (catalog no. S32356, Molecular Probes, Eugene, Oregon).
Non-monocytes were indirectly magnetically labeled with a cocktail of biotin-conjugated mouse monoclonal antibodies against CD3, CD7, CD16, CD19, CD56, CD123 and CD235a, as primary labeling reagents, and anti-biotin monoclonal antibodies that were conjugated with MicroBeads, as secondary labeling reagents.
Mrs. Profeta, 21, is a sales associate in the Manhattan showroom of Viewmark USA, a handbag maker and wholesaler, whose primary label is Chinese Laundry.
Universal, via either its primary label or its Focus and Rogue specialty divisions, already releases about 30 films a year — a full slate.
Mr. Kleck's primary label is Silver Stone, and every so often a chardonnay under that brand arrives in the East End market.
A major drawback of internalizing monoclonal antibodies (mAbs) radioiodinated with direct electrophilic approaches is that tumor retention of radioactivity is compromised by the rapid washout of iodo-tyrosine, the primary labeled catabolite for mAbs labeled via this strategy.
As I see it, the issues here are: (i) the fourth primary system: what is it and why is it relevant in Figure 3? (ii) the first three primary labels listed as simultaneous truly speaking are not systems of field, tenor and mode, since they have no options; what is their function here?
