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Here, we combined the corrected primary alignment (e.g. aligning sequences from each helix only) with the molecular morphometrics data.
Rates of urethral stenosis and fistula were highest after primary alignment.
Of these injuries, 83 (53%) were managed with immediate repair; 17/83 (20%) via primary alignment and 66/83 (80%) via anastomotic repair.
The primary alignment was obtained using ClustalW [47] and corrected by taking the conserved interactions of the lipocalin family (as described in [34]) into account.
It is notable that our method correctly distinguished main domains from swapped domains for almost all of these cases, such that the primary alignment of every DSCO pair was generally based on the main domains.
The primary alignment for each read was used in counting.
Similar(33)
For these tests, we compare our results to BWA-SW which is the most widely used long-read aligner and the most challenging competitor to YAHA for finding primary alignments on either chimeric or non-chimeric queries.
We called SNVs and Indels in WGS and WES using Bambino57, initially requiring that all reads were primary alignments and had a mapping quality of at least 1.
We now compare the accuracy of YAHA to BWA-SW in finding primary alignments.
BWA-SW only reports primary alignments so cannot be included in the sensitivity comparison.
Primary alignments of codons were performed with PRANK (Loytynoja and Goldman 2005) and then visually inspected.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com