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This situation is different for deleterious short-distance tandem splice sites that preserve the reading frame.
Next we checked the proportion of these possible protein-coding exons which preserve the reading frame.
Neither constitutively spliced nor RIs tend to preserve the reading frame.
Compensatory indels involve two or more indels that combine to preserve the reading frame.
DMD is usually caused by deletions, duplications or nonsense mutations that disrupt or truncate the reading frame, while BMD mutations usually preserve the reading frame [ 13, 15].
Thus, the transcript will preserve the reading frame when the length difference of the two exons is an exact multiple of 3 nt in the ME group.
From the analysis of fungal RIs, we found no tendency to preserve the reading frame, similar to results on non-fungal species in a previous study.
In order to preserve the reading frames of CDS, we aligned the amino acid sequences and then back translated them into DNA alignments.
"The real focus for the industry as a whole is how we preserve time for reading," said Tamblyn.
In addition, all recombination generated sequences preserve the polyprotein reading frame, which is not expected in PCR-mediated recombination where selection cannot purge deleterious mutants.
Hexanucleotide repeats also preserve reading frames, but we did not discover any of these among our Stenogyne ESTs.
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