Besides nine genes present in both result files, another 14 genes were identified from 10-kB regions only, with a median methylation change up to 45%% (see Additional file 5).
Additionally PCR reactions were performed using genomic DNA from the male thalli as a template which revealed that all the three genes are also present in both male (Additional file 1: Table S3, Additional file 3: Figure S2) and female genomes.
Next, we analyzed the 20 most strongly expressed genes between the two treatments (CK vs. JA and CK vs. MeJA) and identified nine up-regulated genes and four down-regulated genes present in both treatments (Additional file 15).
No genes related to the synthesis of either β-1,6 or β-1,4-glucans were identified, although hydrolase orthologs for the three types of β-glucan linkages in the S. schenckii cell wall, are present in both genomes (Additional file 11: Table S9).
For each replicon, genes commonly present in both the Protein Table files and the Prodigal files were targeted to minimize false positive gene selection.
Because our version of hospital discharge data did not contain names, we linked them with the population registry using PHNs that were present for all records in both files to retrieve the surname and first name from the population registry.
In the absence of Wip1, STAT5 protein is still present in both cell populations (Additional file 5D), but a conspicuous absence of phosphorylated STAT5 is observed in the ER-positive cells.
A variety of ATP transport systems, often in operons, are present in both chromosomes (see Additional file 1: Table S6) for active uptake of various substrates, such as cations and anions, nucleosides, amino acids, sugars, glycolipids and polyamines.
Analysis of the male and female C. stygia antennal transcriptomes identified three candidate SNMPs present in both data sets (Additional file 2: Table S4 [GenBank accession numbers KJ702172-KJ702174]), twofof which (CstySNMP1 and CstySNMP3) likely represent full-length genes.
