Another filtering was done with a preliminary tree built using FastTree 2.1 [ 34] to identify very similar sequences; only one member of each set of similar sequences was kept.
It is important to point out that eight of the 45 annotated were not represented in the preliminary peptide build at VectorBase.
The S. purpuratus genome sequencing is in progress; therefore, the preliminary assembly (build 1.1) [ 51] may not include all GH18 family genes.
Preliminary budgets are built on best guesses, and the mayor and the City Council have until July to negotiate a final plan.
The preliminary models were built and refined with the Phenix program.
The chapter presents preliminary interface designs built on content supported by a prototype mediator-based system architecture.
After a preliminary contig was built (see below for methods) ten BACs were selected for BAC end sequencing (T7 and Sp6 primers).
To accelerate the establishment of a complete BAC contig, we took advantage of the availability of preliminary BAC contigs built after the fingerprinting of 72 190 BAC of the INRA library (Chardon et al, in preparation).
This preliminary model was built by comparing the respective sites identified by site-directed mutagenesis, manually assembling the two surfaces in the most complementary orientation followed by energy minimisation of the complex.
Our preliminary MRT is built on the basis of a literature review and we further refined it through Key Informant (KI) interviews with policymakers and researchers in the health sector in Uganda.
