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For the in vivo studies, Gd-DTPA tagged compounds were infused into the non-human primate putamen, via an implanted multiport catheter connected to a MedStream™ pump, programmed to deliver a predetermined volume with alternating on off periods to take advantage of the convective and diffusive contributions to Vd.
VGD/DNA polyplexes were formed by adding predetermined volume of VGD to achieve the desired N/P ratio and mixed by vigorous vortexing for 10 s.
After 4-8 minutes, dispense the desired number of cells in the predetermined volume of medium into the 384 well plate.
Tissue sampling is performed with needles that collect a predetermined volume of the tissue of interest.
The powder sample was in every case dispersed into a predetermined volume of the base fluid to obtain the desired weight fraction.
The operation of the cathodic working chamber was conducted as follows: the predetermined volume of fermentation broth was discharged, and the same amount of fresh AGS was added by using a timer and peristaltic pumps (RP2000, EYELA, Tokyo, Japan).
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Although we express financial incentives as a price increase, a more politically feasible option might be to offer cash rebates to households that decrease their volumetric water use from prior years or use less than some predetermined threshold volume.
Mice were monitored for tumor growth and were sacrificed when the tumors reached the predetermined threshold volume (1300 mm).
Furthermore, although we predetermined renal volume and renal function as a secondary outcome, the inclusion of exclusively ADPKD patients allows us to evaluate an effect of lanreotide on kidney volume and function.
On the other hand, there are predetermined limitations for their mass and volume budgets owing to the fact that most of them are launched as an auxiliary payload in which the launch cost is reduced considerably.
In the healthy group, to standardize site selection and obtain adequate fluid volume, sampling was predetermined to be from the mesiobuccal region of the maxillary right first molar, in the absence of which the left first molar was sampled.
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