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Several post-Sanger sequencing technologies (also referred to as next-generation sequencing technologies) have since been developed that enable extremely rapid whole-genome sequencing and a broader application of comparative genomics [ 33, 34].
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The new methods allow an increase in generated sequence per run of about two orders of magnitude compared with conventional Sanger sequencing technologies.
Many well-known metagenomics projects use the whole genome shotgun sequencing approach in combination with Sanger sequencing technologies.
We carried out a three-way comparison of NGS and Sanger sequencing technologies and found relatively low discrepancy rates.
New sequencing technologies are also known as next-generation sequencing (NGS), which makes reference to the first generation of Sanger sequencing technology.
In past sequencing projects using Sanger sequencing technology the main bottleneck was the sequencing itself.
The 4.6-Mbp complete Y. pestis CO92 genome was sequenced by the Wellcome Trust Sanger Institute [36] using Sanger sequencing technology.
Recently developed massively parallel "next-generation" sequencing technologies have begun to replace the previously dominant Sanger sequencing technology [1] [3] for large-scale sequencing projects.
Amplicons were sequenced in a commercial facility using Sanger sequencing technology (Macrogen Europe, Amsterdam, The Netherlands).
The maize genome was completed in 2009 using traditional Sanger sequencing technology, [ 11].
Sanger sequencing technology is used in some initial metagenomic projects [ 4, 5].
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