Positive amplification was observed using a fluorescent detection reagent.
Positive amplification was achieved only with DNA from aflatoxigenic A. flavus and A. parasiticus.
Positive amplification of 896 bp fragments specific for aacC2 genes were observed by PCR designated for the detection of the aminoglycoside resistance genes.
Positive amplification of the genes was confirmed by sequencing.
Positive amplification only with O. cf. ovata cultures demonstrated the species-specificity of primers.
Positive amplification was found for most (234/240) nymphs examined.
Positive amplification was shown by the specific ladder-like pattern on a UV transilluminator at 320 nm.
For positive amplification samples with no inhibitors, concentrations were determined using the standard curve previously developed.
The detection level (DL) was determined as the lower concentration with positive amplification but a CV larger than 0.3.
The results showed that positive amplification was only observed in N. albiflora.
An increase in the ratio of fluorescein to Cy5 fluorescence during the cycling was indicative of positive amplification event.
