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Although organotypic models have fully differentiated keratinocytes, they also have limitations as wound healing is affected both by tissue architecture and the participating cell types [53].
For all subplots, spikes in red during spiking events represent the first spike for each participating cell; all the other spikes are in yellow.
Clearly this would entail loosening of adhesions between all participating cell layers, as well as the matrix substratum, and so our observations add weight to this being the mechanism for sheet migration in stratified epithelia.
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The participating cells contain information needed to develop everything "downstream," but can never become more proximal structures.
(2014) show that epithelial cell clusters secrete FGFs into a microlumen, restricting FGF localization so that participating cells coordinate differentiation and collective migration via luminal signaling.
Genetic inhibition of microlumen assembly and laser micropuncture experiments demonstrate that microlumina increase signalling responses in participating cells, thus allowing FGF to coordinate the migratory behaviour of cell groups at the tissue rear.
The differentiation of xylem culminates in the death of the participating cells, and the vessels are formed of chains of empty walls.
Cell viability within the bio-engineered organ constructs is an essential parameter reflecting the performance of participating cells during long-term ex vivo culture, and is a prerequisite for further functional performance.
And for LC-RB, all the participating cells can work sequentially in order to complete the scheduling.
At each stage, the quality and utility of the information available to the participating cells is being assessed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com