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However, as we did not select subsets for our trafficking studies or our cytotoxicity assays, we do not know whether those cells eliciting in vitro cytotoxicity were those that migrated to the tumour sites.
A distance of 1 μm approximates 1% of the diameter of a cultured astrocyte (Potokar et al., 2005), and, within the time elapsed (40 s) in our trafficking experiments, it is a distance that cannot be attained by simple diffusion of an 80 nm particle in cytoplasm (Luby-Phelps, 2000), which is approximately the size of trafficking vesicles identified in astrocytes (Crippa et al., 2006).
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