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The expression levels and purification protocols were not individually optimized at this stage so some E. coli contaminant proteins common to all purifications independent of bait or PB2 region were observed.
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NCRAWL has been optimized at each stage of the NC operations to utilize the router resources efficiently.
A subsequent meta-analysis by Kern and Shoemaker found a significantly lower mortality in patient groups optimized at an early stage (namely surgical patients optimized peri-operatively), but no formal quality analysis was presented [ 5].
Temperature, moisture content and pH for two astaxanthin producer yeasts, ATCC 24,202 (Xanthophyllomyces dendrorhous) and ATCC 24,259 (Sporidiobolus salmonicolor) were optimized at the first stage.
We finally discuss how some of these parameters can be optimized at the design stage to produce bio-inspired protective systems with desired combination of surface hardness, stability and flexural compliance.
Study quality can be optimized at the design stage by specifying procedures to minimize inconsistencies in trial conduct.
Reviewer 2: Rob Knight, University of Colorado In this manuscript, the authors investigate the hypothesis that the genetic code was optimized at an earlier stage of evolution in which only the first two codon positions were used for coding.
The process is optimized at two stages.
The results from this stage of study encouraged us to proceed further to second stage, with the coagulant dosage optimized at 0.1, 0.2, 0.3 and 0.4 g/l.
However, the objective was not to develop parameters for scaling up the process but rather to optimize SO2 solubility and Pt selectivity at this stage.
Although the enhancers contained in the different lacZ reporters used in our experiments are all clearly capable of communicating with the promoter it would certainly be expected that this communication would be less efficient than for endogenous loci that contain the full complement of flanking DNA that has evolved to optimize the regulation of gene expression at this stage.
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