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An algorithm in three stages is built in order to optimize the procedure of discretization.
It can be used to optimize the procedure of utilizing ammonia as a fuel in future equipment design.
Atomic force microscopy analysis allowed to optimize the procedure of nanoparticle cluster array fabrication in terms of electron beam dose and chemical protocol, enabling to achieve a good regularity in spacing and size (i.e. area and layer number) of the clusters.
Moreover, several modifications were proposed to optimize the procedure of intersection analysis.
Many algorithms have been proposed to optimize the procedure for election of CH.
Several parameters were assayed to optimize the procedure of the previous VFAE protocol (Gomaa et al. 2017).
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This designed study was used to optimize the procedures for the existing methods.
Although several parameters have been determined to optimize the procedures for obtaining a stable and high-frequency mutation, numerous false-positive clones still appear on the plate, which increases the difficulty of finding the desired mutants.
The objective of this study was to optimize the operation procedures for the isolation of adipose stem cells and tissues.
By optimizing the procedure we found the average level of carry-over after transfer of two pronuclei is less than 2.0%, with many of the embryos containing no detectable donor mtDNA.
As a method to deliver TFs into cells, we used synthetic mRNAs prepared by in vitro transcription (Supplementary Fig. S2a) based on our previous experience with mouse ESCs.21 After optimizing the procedure as described in the methods section, we used the method to transfect hESCs with synthetic mRNAs four times during the first two days (Supplementary Fig. S2b).
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