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The resolution performance of a sensor with submicron pixels exceeds the optical resolution limit.
The pixels marked 'a', 'b', and 'c' do not suffer from aliasing because the optical resolution limit is 0.25fs due to diffraction.
The optical resolution limit for these images was 0.48 µm in the XY direction.
The optical resolution limit for these images was 0.13 µm in the XY direction and 1 µm in the Z direction.
This is convenient for applications involving fluorescence microscopy, because vesicles of several microns are ideal for imaging, as the optical resolution limit is ∼0.25 to 0.5 µm for conventional microscopes.
In both cases, particle size values measured by STED imaging were well below the classical optical resolution limit.
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We simulate the optical maximum resolution limit determined by the diffraction of an ideal (aberration-corrected) lens.
However, these bundles/aggregates are beyond the standard optical microscope resolution limit.
Unfortunate optical resolution is limited by diffraction and dispersion effects.
In addition, we use Airyscan super-resolution microscopy (~140 nm-resolution) to bring the optical resolution beyond the diffraction limit of light16,17,18.
Optical resolution beyond the diffraction limit can be achieved by use of a metallic nanoaperture in a near-field optical system.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com