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Although direct culture of monkeypox virus from tissues of these 2 animals was not attempted, the authors noted difficulty detecting monkeypox virus DNA by standard polymerase chain reaction (PCR) and by PCR followed by restriction-endonuclease fragment length polymorphism, even though viral antigen was easily detected by IHC in the same tissue samples (2 ).
Prenatal exposure to permethrin in air and/or blood was not associated with altered neurodevelopment, although the authors noted difficulty measuring permethrin in these media (Horton et al. 2011b).
Previous studies have noted difficulty in engaging the community.
Participants also noted difficulty finding sufficient labor to adequately meet the manual labor demands during the growing season.
During the course of the study, researchers noted difficulty in contacting clinics and began recording the date of each additional attempt required to secure an appointment.
Focus group participants frequently noted difficulty finding and checking out books using call numbers.
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It is not difficulty to understand the increasing char reactivity (the left part of peak) as the Na concentration increased.
Why did the HVN intervention appear to affect bathing difficulty but not difficulty for the other five ADLs?
Mallon et al. reported that difficulty maintaining sleep, but not difficulty initiating sleep, was associated with the risk of diabetes.
Croz, an experienced guide, and Young Peter Taugwalder scouted ahead and returned with the news that there was "not a difficulty, not a single difficulty!..
That isn't to say there aren't difficulties, of course.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com