Newly replicated protozoa present single structures, which includes a unique symbiotic bacterium (Fig. 5A).
When coupled to BrdU (5-Bromo 2-deoxy-uridine) labeling of newly replicated DNA, the replication properties of different DNA sequences can be analysed.
Frum et al. (2008) observed replication pausing near newly replicated origins in early S phase in human fibroblasts.
In a parallel study, we compared newly replicated chromatin with post-replicative chromatin using quantitative proteomics (Alabert et al, 2014).
NSP Nuclear Shuttle Proteinn) acts to import the virus genome into the nucleus for replication, and to export newly replicated virus genomes back to the cytoplasm.
These results, together with the increased accumulation of γ-H2AX foci at sites of newly replicated DNA, indicate the presence of replication stress.
Hansen, R. S. et al. Sequencing newly replicated DNA reveals widespread plasticity in human replication timing.
All herpesviruses encode an AE, which is critical for viral lytic replication, assisting in the processing of newly replicated viral genomes [7].
As DNA replication takes place, some of the newly replicated minichromosomes are committed to transcription through the binding of specific and general transcription factors.
These interactions are pivotal for maintaining accurate replication of histone methylation patterns in newly replicated chromatin and in the subsequent fidelity of gene expression.
It is generally believed that all newly replicated DNA is reassembled into chromatin following DNA replication.
