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Multiplex sequencing of 16S rRNA tags revealed that both types of RS induced several significant compositional alterations in the fecal microbial populations, with differential effects on community structure.
Multiplex sequencing has also been accomplished by tagging randomly sheared DNA fragments from different samples with unique, short DNA sequences (barcodes) and pooling samples into a single sequencing channel [14].
Multiplex sequencing of 101 bp paired-end reads was performed on an Illumina HiSeq2500 instrument.
Multiplex sequencing of transcript 3′ ends identifies differential transcript abundance independent of gene annotation.
We established the high throughput, high resolution, and high fidelity HLA genotyping using transposase-based library construction and multiplex sequencing with MiSeq sequencer.
The library was subjected to multiplex sequencing on the MiSeq sequencer (Illumina).
Equal volumes of libraries were pooled and subjected to multiplex sequencing on the MiSeq sequencer (Illumina).
PCR amplicons were subjected to transposase-based library construction and multiplex sequencing with the MiSeq sequencer.
We applied long-range PCR to amplify HLA-B for 96 samples followed by transposase-based library construction and multiplex sequencing with the MiSeq sequencer.
A library-specific barcode for multiplex sequencing was part of a 3′-sequencing adapter.
High-throughput multiplex sequencing was carried out using a Genome Sequencer (GS) FLX Instrument (Roche Diagnostics, USA) and Genome Analyzer II (Illumina Inc., USA) sequencers.
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