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Furthermore, a multiple testing analysis was not applied to genotypic differences in relation to various measures of kidney function.
Limitations of the study include small size of the sample, small number of C/C genotype patients, and a lack of multiple testing analysis of genotypic differences in various measures of kidney function.
MZ was responsible for multiple testing analysis.
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Multi-experiment viewer v4.4 (Mev) software was used for the Significance Analysis of Microarray (SAM) method, a multiple testing statistical analysis, to identify genes that were differentially expressed in response to SSR.
Multiple testing correction analysis was not applied, and all unassigned reads were ignored.
The authors thank Dr. Sabina Bijlsma (TNO, the Netherlands) for help with multiple testing correction analysis.
Analysing the many possible combinations would require very large numbers to allow correction for multiple testing, an analysis beyond the scope of this study.
Without adjustment for multiple testing, multivariate analysis demonstrated an association with increased bladder cancer risk with PARP1 rs8679 (Ptrend = 0.05) while variant homozygotes of PARP1 rs8679 were also noted to have an increased breast cancer risk (P = 0.03).
Although no marker passed stringent Bonferroni multiple testing and further analysis (e.g., haplotype based analysis) and replication is needed, this preliminary data suggests that our candidate gene selection approach might be effective.
The false discovery rate (FDR) was used to determine the threshold of P value in multiple test and analysis.
FDR (false discovery rate) was used to determine the threshold of P-value in multiple test and analysis.
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