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Multiple alignment analysis was performed using Multalin (http://multalin.toulouse.inra.fr/).inra.fr/
Multiple alignment analysis was performed with ClustalX.
Multiple alignment analysis of Trs130-related proteins is shown in Figures 3 and S2.
Multiple alignment analysis was performed on full-length AP2 domain sequences from Hevea, Arabidopsis and Populus.
Multiple alignment analysis was performed with ClustalX and subsequently adjusted manually by Genedoc (Larkin et al. 2007).
Multiple alignment analysis of each TRAPPII-specific subunit revealed conserved domains that include highly conserved amino acids.
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Both genes shared high similarity with their counterparts according to the multiple alignments analysis.
Multiple alignments analysis reveals conserved domains with highly conserved amino acids in each protein family.
Multiple alignments analysis showed that AsCOI1 had more than 70% sequence identity with the COI1 proteins of several other species, which suggested that COI1 proteins were highly conserved.
Approximate locations of TM domains in each family were assessed by a combination of hydropathy plots of multiple alignments, analysis of a number of single family members by TMHMM [ 51], and hand reconciling of different family predictions for related families.
Multiple-alignment analysis of sequences obtained in this study and reference sequences from GenBank (accession nos.
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