Sentence examples for Mrs pcr from inspiring English sources

2 different primer and probe sets were used: methicillin-resistant staphylococcus (MRS) PCR using a methicillin-resistant Staphylococcus aureus (MRSA) detection kit (Roche Diagnostics GmbH, Mannheim, Germany) and broad-range universal PCR, as previously described (Kobayashi et al. 2006, 2009).

Results were compared with those obtained using uniform priors for mr-PCR to test robustness.

Multiplex real-time PCR (mr-PCR) enables testing the presence of several bacteria and reduces diagnosis time.

The mr-PCR was designed to simultaneously detect Staphylococcus aureus, Streptococcus uberis, Escherichia coli, and Streptococcus agalactiae.

Multiplex real-time PCR Se on milk from clinical mastitis were lower than mr-PCR Se on milk from normal quarters.

Milk samples from 1,014 clinical mastitis cases and 1,495 samples from apparently normal quarters were analyzed by BC and mr-PCR.

Differences in sample age between conduct of BC and of mr-PCR did not concur with any apparent differences between Se estimates of the 2 tests.

A significant association was observed between sample age and mr-PCR results leading to reduced detection of E. coli and Strep.

Our objective was to estimate bacterial species-specific sensitivity (Se) and specificity of both BC and mr-PCR tests for detecting bacteria in milk samples from clinical mastitis cases and from apparently normal quarters, using a Bayesian latent class model.

The 777-bp bioHx structural gene (not including the stop codon) was amplified with primers BioHx-MF and BioHx-MR, the purified PCR product was digested with BamHI and HindIII, and then cloned into the expression vector pET-28a at BamHI and HindIII sites.

LV and MR carried out the PCR assays, cloning and sequencing work.