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The two stage system degraded 6% more solids than the single stage system.
The SI-35 treatment required more solids removal, oxygen, and bicarbonate supplementation than the HI-35 treatment.
The mass ratio of solids to water is higher in conventional cell compared to the flotation column, which indicates that the shallow froth depths push more solids to the froths compared to the deep froths.
When more solids were used, higher amounts of cellobiose were released and enzymes inhibited yielding lower amounts of sugars especially in hydrolysates containing 22% solids.
The adsorption decreases with increasing solids content, and at 30% solids content only approximately 17% of the added enzyme is adsorbed, despite significantly more solids remaining than at lower solids contents.
After no more solids could be removed, the aqueous fraction of each sample was sequentially filtered through 2.0 and 1.2 μm pore size glass fiber filters (Millipore, 47 mm diameter), and lagoon solids were freeze-dried to dryness.
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